Image Acquisition, Reconstruction, and Presentation

DOWN-LOADABLE DATA SETS

3-D Reconstruction 2008-01-10 from 85 Projections

3-D Reconstruction 2008-01-10 from 85 Projections

Quality Control Display: Original versus Re-Projection of the Reconstructed Model and Model Residuals

Note: The mismatches indicated by the residuals at #5 and #85 indicate:

1. the logarithmic scaling constants are not correct (slow waves in the residuals)

2. the shrinking of the sample thickness is not fully corrected by the current regression model

of the beads (off-center patterns of the beads)

Original (trimmed at 4-sigma level) | Re-Projected (trimmed at 4-sigma level) | Residual (trimmed at 4-sigma level) | |
---|---|---|---|

Projection #5 | |||

Projection #45 | |||

Projection #50 | |||

Projection #85 |

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Note: Double-Tilt, High Density is Black on IMOD

Note: Double-Tilt, Zero-Density is Black on IMOD, 3-rd order Polynomial

Original (trimmed at 4-sigma level) | Re-Projected (trimmed at 4-sigma level) | Residual (trimmed at 4 sig) | |

Projection
#46 at (0,0) deg |

3-D Reconstruction 2008-05-21 from 166 Projections (ca. +/- 60º in A-series and +/- 70º in B-series)

Note: Double-Tilt, Zero-Density is Black on IMOD, 4-th order Polynomial

3-D Reconstruction 2008-05-03 from 49 Projections (+/- 72 degree range, 3º increments)

Note: Single Tilt, some 100 cubes, each 6x6x6 voxels, x/y/z-axes new standard, base-density removed

Maria's Simulation: tom_0_48_3

3-D Reconstruction 2008-05-01 from 3 Projections (-72, 0, 72 degrees; subset of above data, 72º increments)

Note: Single Tilt, some 100 cubes, each 6x6x6 voxels, base-density removed

Maria's Simulation: tom_0_48_72

Original (not trimmed) | Re-Projected (not trimmed) | Residual (not trimmed) | |

Projection #24, 0 deg |

3-D Reconstruction 2008-05-15 from 49 Projections (+/-72º range with 3º increments)

Note: Single Tilt, some 100 cubes, each 6x6x6 voxels, with base density, density up-scaling factor is 200

estimated background density is 0.80, estimated cube density is ca. 5.0

Maria's Simulation: tom_0_48_3

Origina Projection Data (Windowed) | Predicted Projection Data | Residual Projection Data | |

Projection at -60 deg | |||

Projection at 0 deg | |||

Projection at 60 deg |

Above images are a selected from a 9-View Reconstruction of 1024x1024 pixel projections (half original resolution).

The apparent vertical "edges" within the -60 and +60 deg images (ca. 30% from the dark borders - relative to the displayed sample size)

arise from the artifactual "cutting" of the 3-D object space into a volume that is contained in all projections (edges of the sample slab).

Alice Dohnalkova's 9-View Reconstruction of Manganese Sample

Alice Dohnalkova's 17-view Reconstruction of Manganse Sample

Alice Dohnalkova's 41-view Reconstruction of Manganse Sample

Recommended Resolution for Viewing is 1600x1200 pixels